Drug Target Identification in Saccharomyces cerevisiae Using DNA Microarrays

Patrick Flaherty and Adam Arkin1
(Professor Michael I. Jordan)
(ONR) N00014-00-1-0758

In recent years, high throughput biological techniques have been developed to collect data on the genomic response of an organism to stress. We employ DNA microarray technology to gain a survival snapshot of haploinsufficient Saccharomyces cerevisiae (bakers' yeast) in the presence of several antifungal drugs, anticancer drugs, and environmental stresses. Using well-known statistical and machine learning techniques, we identify protein targets and side effects of these stresses with a measure of confidence. This information is further used to hypothesize previously unknown protein functions, molecular and genetic pathways, and drug interactions.

G. Giaever et al., "Functional Profiling of the Saccharomyces cerevisiae Genome," Nature, July 2002.
1Outside Adviser (non-EECS)

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