Drug Target Identification in Saccharomyces cerevisiae Using DNA Microarrays

Patrick Flaherty and Adam Arkin1
(Professor Michael I. Jordan)
(ONR) N00014-00-1-0758

In recent years, high throughput biological techniques have been developed to collect data on the genomic response of an organism to stress. We employ DNA microarray technology to gain a survival snapshot of haploinsufficient Saccharomyces cerevisiae (bakers' yeast) in the presence of several antifungal drugs, anticancer drugs, and environmental stresses. Using well-known statistical and machine learning techniques, we identify protein targets and side effects of these stresses with a measure of confidence. This information is further used to hypothesize previously unknown protein functions, molecular and genetic pathways, and drug interactions.

[1]
G. Giaever et al., "Functional Profiling of the Saccharomyces cerevisiae Genome," Nature, July 2002.
1Outside Adviser (non-EECS)

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